硫酸耐尔蓝
生物活性体外研究 用途与合成方法 MSDS 硫酸耐尔蓝价格(试剂级) 上下游产品信息
中文名称 | 硫酸耐尔蓝 |
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中文同义词 | 硫酸耐尔蓝;硫酸尼罗蓝;耐尔蓝;耐尔兰A;尼罗蓝A;尼罗蓝硫酸盐;耐尔蓝A*荧光参照标准*;耐尔兰指示液 |
英文名称 | CI 51180 |
英文同义词 | 5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iusulfate(2:1);9-(diethylamino)-5-imino-5h-benzo(a)phenazoxazinsulfate(2:1);Benzo[.alpha.]phenoxazin-7-ium,5-amino-9-(diethylamino)-,sulfate(2:1);nileblueasulphate;BASIC BLUE 12;BASIC BLUE 12 SULFATE SALT;CI NO 51180;CI 51180 |
CAS号 | 3625-57-8 |
分子式 | C20H20N3O5S- |
分子量 | 414.46 |
EINECS号 | 222-832-5 |
相关类别 | 色素类;血液学和组织学;染色剂和染料;生化试剂;化学试剂;生化试剂-色素类;通用生化试剂-生物染色剂与化学指示剂;Phenoxazine;marker |
Mol文件 | 3625-57-8.mol |
结构式 |
硫酸耐尔蓝 性质
熔点 | >300 °C (dec.)(lit.) |
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储存条件 | room temp |
溶解度 | 易溶于水、乙醇 |
色指数 | 51180 |
形态 | 粉末 |
颜色 | 深绿色到黑色 |
酸碱指示剂变色ph值范围 | 10.1(BLUE)-11.1(RED) |
水溶解性 | Soluble in ethanol, and water (50 g/L) at 25°C. |
最大波长(λmax) | 633 nm |
生物领域应用 | Detecting microorganisms; treating virus infectious diseases; photodynamic therapy |
硫酸耐尔蓝 用途与合成方法
生物活性
Nile Blue A (Nile blue sulfate) 可用于区分黑色素和脂褐素。它也可用于脂肪染色,以及制备电流葡萄糖传感器。
体外研究
Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein. Nile blue A is one of the most studied benzophenoxazine dyes, as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule.
用途
生物染色,区别脂肪酸和中性脂肪,病理组织中细菌和放线菌染色。也用于钽的光度测定。在中和分析中用以制备混合指示剂。
安全信息
危险品标志 | Xi |
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安全说明 | 22-24/25 |
WGK Germany | 3 |
RTECS号 | DJ1931000 |
海关编码 | 3212900000 |
毒性 | mouse,LD,intraperitoneal,> 500mg/kg (500mg/kg),"Summary Tables of Biological Tests," National Research Council Chemical-Biological Coordination Center. Vol. 4, Pg. 46, 1952. |
MSDS信息
提供商 | 语言 |
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ACROS | 英文 |
SigmaAldrich | 英文 |
ALFA | 中文 |
ALFA | 英文 |
硫酸耐尔蓝 价格(试剂级)
更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
---|---|---|---|---|---|
2024/08/19 | A17174 | 耐尔兰 Nile Blue A | 3625-57-8 | 5g | 348元 |
2024/08/19 | A17174 | 耐尔兰 Nile Blue A | 3625-57-8 | 25g | 752元 |
硫酸耐尔蓝 上下游产品信息
上游原料
5-(二乙氨基)-2-(亚硝基苯酚)1-萘胺