BETA-淀粉样蛋白片断25-35 CAS#: 131602-53-4

微信扫码

BETA-淀粉样蛋白片断25-35

生物活性体外研究体内研究 用途与合成方法 MSDS BETA-淀粉样蛋白片断25-35价格(试剂级) 上下游产品信息

中文名称	BETA-淀粉样蛋白片断25-35
中文同义词	BETA-淀粉样蛋白片断25-35;淀粉样Β-蛋白片段25-35;AMYLOID Β-PROTEIN FRAGMENT 25-35 Β-淀粉状蛋白25-35;BETA-类淀粉蛋白肽(25-35);Β-淀粉状蛋白25-35;Β-AMYLOID (25-35);Β-淀粉状蛋白25-35;Β淀粉样蛋白25-35
英文名称	AMYLOID BETA-PROTEIN (HUMAN, 25-35) TRIFLUOROACETATE
英文同义词	Amyloid beta-peptide(25-35);AMYLOID BETA-PROTEIN (HUMAN, 25-35) TRIFLUOROACETATE;H-GLY-SER-ASN-LYS-GLY-ALA-ILE-ILE-GLY-LEU-MET-OH;AMYLOID BETA-PEPTIDE (25-35) (HUMAN);AMYLOID BETA-PROTEIN FRAGMENT 25-35;AMYLOID BETA-PROTEIN (25-35);AMYLOID-BETA PROTEIN (25-35), HUMAN, MOUSE, RAT;Amyloid beta-Protein (25-35) trifluoroacetate salt
CAS号	131602-53-4
分子式	C45H81N13O14S
分子量	1060.27
EINECS号
相关类别	细胞生物学;多肽;细胞信号和神经生物学;多肽，氨基酸;其他生化试剂;生化试剂-蛋白质;目录肽;目录多肽;proteins;Alzheimers  and  Neurodegenerative  Disease  Research;Amyloid  beta  Protein  Fragments;Amyloid  β  Protein  FragmentsNeuropeptides;Peptide;Neurodegenerative  Disease  Peptides;β  Amyloid  Peptides;Amyloid beta-peptide and related;Alzheimer
Mol文件	131602-53-4.mol
结构式

BETA-淀粉样蛋白片断25-35 性质

BETA-淀粉样蛋白片断25-35 用途与合成方法

生物活性

β-Amyloid (25-35) (Amyloid beta-peptide (25-35)) 是阿尔茨海默氏淀粉样蛋白β肽的Aβ(25-35) 片段，在培养细胞中显示出神经毒性活性。

体外研究

The amino acid sequence of Aβ(25-35) peptide is NH     ₂    -Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-COOH, where the first Gly represents the amino acid 25 and the last Met represents the amino acid 35. Amyloid beta-peptide(25-35) is also investigated in gel state for the first time. Comparative studies are also carried out using vibrational absorption and ECD. The conformational preference of Aβ(25-35) peptide film is also investigated using vibrational absorption and VCD spectroscopy. Amyloid beta-peptide(25-35) induces apoptotic effects on isolated brain mitochondria and the redox state of methionine-35, plays a key role in the induction of programmed cellular death pathways and toxic events.  
 β-Amyloid Aggregation Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).  
 1. Solid Aβ peptide was dissolved in cold hexafluoro-2-propanol (HFIP). The peptide was incubated at room temperature for at least 1h to establish monomerization and randomization of structure.  
 2. The HFIP was removed by evaporation, and the resulting peptide was stored as a film at -20 or -80 ℃.  
 3. The resulting film was dissolved in anhydrous DMSO at 5 mM and then diluted into the appropriate concentration and buffer (serum- and phenol red-free culture medium) with vortexing.  
 4. Next, the solution was age 48h at 4-8 ℃. The sample was then centrifuged at 14000g for 10 min at 4-8 ℃; the soluble oligomers were in the supernatant. The supernatant was diluted 10-200-fold for experiments.  
 Methods vary depends on the downstream applications.

体内研究

Rats are injected with Aβ25–35 peptide intracerebroventricularly and compound Danshen (CDS) are subsequently administered once daily for 23 days. Rats’ behavior is monitored using Morris water maze and passive avoidance. Real time PCR and Western blotting are used in determining amyloid precursor protein (APP), β-site APP cleaved enzyme-1(BACE1), Presenilin-1 (PS1), Insulin-degrading enzyme (IDE) and neprilysin (NEP) in hippocampus. The Alzheimer' s disease (AD) model group present with spatial learning and memory impairments. CDS and donepezil administration significantly ameliorate the Aβ25–35 peptide-induced memory impairment in both Morris water maze (P     [3]    .

参考质量标准

外观:白色粉末
纯度(HPLC) ≥98.0%
醋酸根含量5.0%～12.0%
水分含量≤8.0%
肽含量≥80.0%

用途 

Amyloid β的功能区。兼具有神经营养和神经毒素效果。用于分子生物学研究。

安全信息

MSDS信息

BETA-淀粉样蛋白片断25-35 价格(试剂级)

BETA-淀粉样蛋白片断25-35 上下游产品信息