别名: CCK-1 CCL28 Mucosae-associated Epithelial Chemokine
产品名称
Biochem/physiol Actions
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Physical form
Lyophilized with no additives.
General description
MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant human MEC is a 12.3 kDa protein containing 108 amino acid residues.
Sequence
SEAILPIASS CCTEVSHHIS RRLLERVNMC RIQRADGDCD LAAVILHVKR RRICVSPHNH TVKQWMKVQA AKKNGKGNVC HRKKHHGKRN SNRAHQGKHE TYGHKTPY
性质
| recombinant | expressed in E. coli |
| assay | ≥98% (HPLC) |
| ≥98% (SDS-PAGE) | |
| form | lyophilized |
| mol wt | mol wt 12.3 kDa |
| impurities | <0.1 EU/μg endotoxin, tested |
| suitability | cell culture tested |
| UniProt accession no. | Q9NRJ3 |
| shipped in | wet ice |
| storage temp. | ?20°C |
| Gene Information | human ... CCL28(56477) |
