The universal, silica-based bonded phase used for the ACQUITY HSS T3 sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The ACQUITY HSS T3 UPLC columns exhibit ultra-low MS bleed and are fully scalable and transferable to HPLC separations. The VanGuard pre-column connects directly to the inlet of the ACQUITY UPLC column which minimizes extra columns volumes and reduces leaking by eliminating additional connections.