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• Brand

• BD Pharmingen™

• Application

• Immunocytochemistry, Intracellular staining (flow cytometry), Immunofluorescence, Flow cytometry (Tested During Development)   
  

• Storage Buffer

• Lyophilized powder

• Regulatory Status

• RUO

Regulatory Status Legend

• RUO

• For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• RUO (GMP)

• For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

• GPR

• General Purpose Reagent

• IVD

• For In Vitro Diagnostic Use.

• ASR

• Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

BD Pharmingen™ Hoechst 34580 is a reagent for the fluorescent staining of DNA and nuclei in live or fixed cells. Hoechst 34580 is a bisbenzimidazole dye with high specificity for binding to double-stranded DNA. Since Hoechst 34580 is specific for DNA binding, ribonuclease treatment is not needed to avoid nonspecific RNA staining. In addition to its use in fluorescence microscopy and image analysis, Hoechst 34580 is commonly used for flow cytometric applications, such as cell cycle analysis.

Hoechst 34580 can be excited by either UV (eg, 355 or 375 nm) or violet (eg, 405 nm) light sources, and has an excitation maximum of 368 nm. It emits blue fluorescence with an emission maximum of 437 nm.

Preparation and Storage

Store at -20°C, protected from exposure to light.  

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.