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• Brand

• BD Pharmingen™

• Concentration

• 0.2 mg/ml

• Application

• Flow cytometry (Routinely Tested)   
  

• Storage Buffer

• Aqueous buffered solution containing ≤0.09% sodium azide.

• Regulatory Status

• RUO

Regulatory Status Legend

• RUO

• For Research Use Only. Not for use in diagnostic or therapeutic procedures.

• RUO (GMP)

• For Research Use Only. Not for use in diagnostic or therapeutic procedures. Although not required, these products are manufactured in accordance with Good Manufacturing Practices.

• GPR

• General Purpose Reagent

• IVD

• For In Vitro Diagnostic Use.

• ASR

• Analyte Specific Reagent. Analytical and performance characteristics are not established.

Any use of products other than the permitted use without the express written authorization of Becton, Dickinson and Company is strictly prohibited.

Description

SAv-PerCP is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis.

PerCP is a photosynthetic accessory pigment from Glenodinium species of dinoflagellates, which is excited by the 488-nm light of an Argon ion laser and fluoresces at 675 nm. Therefore, PerCP-labelled antibodies can be used with FITC- and R-PE-labelled reagents in most single-laser flow cytometers with no significant spectral overlap of PerCP fluorescence with that of FITC or R-PE.

Format

• Format

• PerCP

• Excitation Source

• Blue 488 nm

• Excitation Max

• 482 nm

• Emission Max

• 678 nm

PerCP is a component of the photosynthetic apparatus found in the dinoflagellate Glenodinium. PerCP is a protein complex with a molecular weight of approximately 35 kDa. Due to its photobleaching characteristics, PerCP conjugates are not recommended for use on flow cytometers with high-power lasers (>25 mW).

Other Formats →

• AKP

• APC

• APC-Cy™7

• APC-R700

• BB515

• BUV395

• BUV496

• BUV563

• BUV661

• BUV737

• BUV805

• BV421

• BV510

• BV605

• BV650

• BV711

• BV786

• FITC

• HRP

• PE

Preparation and Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. Streptavidin was conjugated with dye under optimum conditions, and unconjugated Streptavidin and free dye were removed  

Product Notices

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.

3. PerCP is a photosynthetic accessory pigment from Glenodinium species of dinoflagellates, which is excited by the 488-nm light of an Argon ion laser and fluoresces at 675 nm. Therefore, PerCP-labelled antibodies can be used with FITC- and R-PE–labelled reagents in most single-laser flow cytometers with no significant spectral overlap of PerCP fluorescence with that of FITC or R-PE. PerCP has been reported to undergo significant photobleaching, the magnitude of which increases as laser power is increased or beam focus is narrowed. For third-color flow¬cytometric analysis using ≥25-mW laser power, we recommend PE-Cy5-, PE-Cy7–, or PerCP-Cy5.5-conjugated reagents.

4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

5. Please refer to http://regdocs.bd.com to access safety data sheets (SDS).

6. Cy is a trademark of GE Healthcare.

7. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.