详细说明
| Replacement Information |
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重要规格表
| Key Applications | Entrez Gene Number | Species | Uni Prot Number |
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| ACT | NM_001223.3, NM_033292.2, NM_033293.2, NM_033294.2, NM_033295.2 | Human | P29466 |
| Description | |
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| Catalogue Number | CC126 |
| Brand Family | Chemicon® |
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| Description | Caspase 1, recombinant human active |
| Overview | Caspase-1 (also known as ICE, Interleukin-1beta-Converting Enzyme) is the prototypical member of the caspase family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa pro-enzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active enzyme is a heterotetramer consisting of two large and two small subunits. To date the mechanism of regulation of caspase-1 activation is complex and poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive pro-enzyme form. The active recombinant human caspase-1 was expressed in E. coli. The expressed caspase-1 spontaneously undergoes autoprocessing to yield the subunits characteristic of the native enzyme. The recombinant caspase-1 preferentially cleaves the substrates consisting of consensus sequence YVAD (e.g., YVAD-AFC and YVAD-pNA). The recombinant enzyme can also be used as a positive control in caspase assays or in determining the specificity of caspase substrates. Recommended usage: 0.5-1 unit for fluorometric caspase assays and 2-4 units for colorimetric caspase assays. Optimal working dilutions must be determined by end user. |
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| Presentation | Lyophilized. Reconstitute in 25 μL PBS containing 15% glycerol. |
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| Biological Information | |
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| Purity | Partially purified recombinant caspase 3 with a full length HIS-6-tag. Approximately 10-20% caspase 1 protein. The remainder is E.Coli proteins. Approximately 0.8-2 mg total protein/U of activity; E.Coli proteins have no reactivity. |
| Entrez Gene Number |
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| Entrez Gene Summary | This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. |
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| UniProt Summary | FUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. SIZE: 404 amino acids; 45159 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain. PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism. SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. |
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| Storage Conditions | Maintain unopened and rehydrated product at -70°C in undiluted, freeze-thaw protected, tightly sealed aliquots. Avoid repeated freeze/thaw cycles. Product stable for 6 months from date of purchased if handled appropriately. |
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| Material Size | 25 units |
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