详细说明
| Replacement Information |
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重要规格表
| Species Reactivity | Detection Methods |
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| A Broad Range Of Species | Fluorometric or colorimetric |
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| Description | |
|---|---|
| Overview | A convenient assay kit useful for measuring the caspase-3 and caspase-3-like activities in cell extracts using either a colorimetric or fluorometric substrate. Provided in a convenient 96-well format with all reagents necessary for preparing cell extracts, measuring caspase activity and calibrating the assay. In addition, caspase-3 is included for use as a positive control, for testing cell extracts for endogenous inhibitors, or for comparing effects of exogenous inhibitors on cellular activity versus the activity of the purified enzyme. |
| Catalogue Number | 235419 |
| Brand Family | Calbiochem® |
| Materials Required but Not Delivered | • plate reader capable of measuring A405 to ≥3-decimal accuracy or fluorescent plate reader capable of reading at an excitation of wavelength of 360 nm and an emission wavelength of 460 nm. • Pipettor or any multi-channel pipettor capable of pipetting 10-100 µl accurately. Note: dilution of reagents can be made to increase the minimal volume to >10 µl. • Ice bucket to keep reagents cold until use. • Apoptosis-induced cultured cells. • Centrifuge • Phosphate buffered saline (PBS). |
| References | |
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| References | Thornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312. Faleiro, L., et al. 1997. EMBO J. 16, 2271. Kothakota, S., et al. 1997. Science 278, 294. Nicholson, D.W. 1996. Nat. Biotechnol. 14, 297. Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841. Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486. Nicholson, D.W., et al. 1995. Nature 376, 37. Tewari, M., et al. 1995. Cell 81, 801. Fernandes-Alnemi, T., et al. 1994. J.Biol. Chem. 269, 30761. |
| Product Information | |
|---|---|
| Detection method | Fluorometric or colorimetric |
| Form | 96 Tests |
| Format | 96-well plate |
| Kit contains | Human Recombinant Caspase-3, Cell Lysis Buffer, a DEVD-pNA Colorimetric Substrate, Ac-DEVD-AMC Fluorometric Substrate, Calibration Standard (p-Nitroaniline), AMC Calibration Standard, an Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol. |
| Applications |
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| Biological Information | |
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| Assay time | 2.5-3 h |
| Sample Type | Cell extracts |
| Species Reactivity |
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| Physicochemical Information |
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| Dimensions |
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| Materials Information |
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| Toxicological Information |
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| Safety Information according to GHS |
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| Safety Information | |
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| R Phrase | R: 23/24/25-63-36/37/38-33 Toxic by inhalation, in contact with skin and if swallowed. Possible risk of harm to the unborn child. Irritating to eyes, respiratory system and skin. Danger of cumulative effects. |
| S Phrase | S: 36/37/39-45-26-23 Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Do not breathe fumes. |
| Product Usage Statements |
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| Storage and Shipping Information | |
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| Ship Code | Dry Ice Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Hazardous Materials Attention: | Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
| Storage | ≤ -70°C |
| Storage Conditions | Upon arrival store the 1/2 volume plate at room temperature and the remaining components of the kit at -70°C. |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
| Packaging Information |
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| Transport Information |
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| Supplemental Information | |
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| Kit contains | Human Recombinant Caspase-3, Cell Lysis Buffer, a DEVD-pNA Colorimetric Substrate, Ac-DEVD-AMC Fluorometric Substrate, Calibration Standard (p-Nitroaniline), AMC Calibration Standard, an Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol. |
| Specifications |
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