详细说明
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重要规格表
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| ChIP-chip |
| Description | |
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| Catalogue Number | 17-1004 |
| Brand Family | Upstate |
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| Description | Magna ChIP2 ™ Universal ChIP DNA Microarray Quad Kit |
| Overview | ChIP-chip (ChIP-on-chip) is a high content approach that combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to analyze how regulatory and structural proteins, such as transcription factors and histone subunits, bind and interact with the genome of living cells. Through the immunoprecipitation of these protein-DNA complexes, associated sequences are pulled out of solution for analysis on DNA microarrays. Using high quality antibodies, post-translational modifications to these sequences can also be detected. The combination of ChIP and promoter or genomic tiling microarrays allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution at a variety of locations. This more comprehensive approach allows for greater insight into gene regulatory networks and can facilitate the creation of genome-wide maps of protein-DNA interactions or histone modifications. Millipore’s Magna ChIP2 ™ Chromatin Immunoprecipitation DNA microarray kits are designed to help address these challenges and make ChIP-chip technology easily accessible to a wider range of laboratories. Each of these user-friendly kits provides a set of optimized reagents and validated protocols to facilitate ChIP-chip for both beginners as well as experienced users. |
| Materials Required but Not Delivered | Labeling and hybridization reagents. User-provided microarray slides. Magna Grip™ Rack 8 well ( 20-400) (Now Available!) or similar magnetic rack. |
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| Presentation | Nine boxes containing all necessary reagents to perform the chromatin immunoprecipitation and amplification steps for 12 ChIP-on-chip assays/ comparison reactions. |
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| Application | ChIP-chip (ChIP-on-chip) is a high content approach that combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to analyze how regulatory and structural proteins, such as transcription factors and histone subunits, bind and interact with the genome of living cells. |
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| Storage Conditions | Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 6 months from date of shipment when stored as directed. |
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| Material Size | 12 assays |
| Material Package | Magna ChIP2 Kit capacity: 12 ChIP-on-chip assays |
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