详细说明
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| Description | |
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| Catalogue Number | UC0E05 |
| Description | UCOE® Intermediate Vector Set |
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| Background Information | The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing. CET 1019 contains the 3 kb mouse Rps3 UCOE and the 1.5 kb fragment of human HNRPA2B1-CBX3 UCOE. CET 1310 contains the 8 kb fragment of human HNRPA2B1-CBX3 UCOE. CET 1316 contains the 4 kb fragment of human HNRPA2B1-CBX3 UCOE. References Antoniou, M., et al.. (2003) Transgenes Encompassing Dual Promoter CpG Islands from the Human TBP and HNRPA2B1 Loci are Resistant to Heterochromatin-mediated Silencing. Genomics, 82: 269-279. Antoniou, M., et al. (2010) A Ubiquitous Chromatin Opening Element (UCOE) Confers Resistance to DNA Methylation-mediated Silencing of Lentiviral Vectors. Mol Ther, 18: 1640-1649. THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com. |
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| Application Notes | Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression by removing epigenetic related gene silencing. |
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| Concentration | Please refer to lot specific datasheet. |
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| Storage Conditions | Plasmids should be stored at -20C. Avoid repeated freeze-thaw events. |
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| Material Size | 1 kit |
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