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| Description | |
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| Overview | A complete mammalian proteome extraction kit (C-PEK) designed to extract virtually all proteins from both mammalian tissues and culture cells in a simple two step process within a microcentrifuge tube to minimize protein loss. The extraction results in a total protein sample that is a clarified and non-viscous protein solution showing higher resolution and an increased total number of spots in 2-D gel electrophoresis. Sample size: 1x107 - 1x108 cells or 50-100 mg tissue. |
| Catalogue Number | 539779 |
| Brand Family | Calbiochem® |
| Synonyms | C-PEK Mammalian Kit |
| Materials Required but Not Delivered | • High purity water, conductivity<5 µS (e.g., Merck) • Cell culture equipment, media etc. for cell growth (e. g., RPMI, DMEM). • Micropipettes and tips, 10 µl, 200 µl and 1 ml size (e.g., Eppendorf, Gilson or equivalent) • Cooled centrifuge and rotor for 50 ml tube size (Eppendorf, Heraeus, Nalgen, etc.) • Cooled microcentrifuge and rotor up to 25,000 x g for 2 ml tube size (Eppendorf, Heraeus, Nalgen etc.) • Thermomixer or rolling facility (e.g., Eppendorf) • Optional: Ultracentrifuge and rotor up to 75,000 x g for 2 ml tube size (e.g. Beckman, Sorvall etc.) • For tissue extraction: Mortar and pestle made from porslin, small spatula. These instruments must be cooled to -70°C or colder prior to be used in the C-PEK procedure! |
| References | |
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| References | Yuan, X., et al. 2002. Electrophoresis 23, 1185. Allen, L. 2000. Functional Genomics Nature 405 819. Herbert, B. 1999. Electrophoresis 20, 660. |
| Product Information | |
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| Form | 20 Extractions |
| Kit contains | Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol. |
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| Biological Information | |
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| Sample Type | Mammalian tissues and cultured cells |
| Physicochemical Information |
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| Dimensions |
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| Materials Information |
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| Toxicological Information |
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| Safety Information according to GHS |
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| Safety Information |
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| Product Usage Statements | |
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| Intended use | ProteoExtract™ Complete Mammalian Proteome Extraction Kit is designed to extract total proteins from mamalian tissue culture cells or mammalian tissue. |
| Storage and Shipping Information | |
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| Ship Code | Ambient Temperature Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | +2°C to +8°C |
| Storage Conditions | • Wash Buffer The Wash Buffer is provided ready-to-use. The buffer should be stored at 4°C. • Resuspension Buffer The Resuspension Buffer is provided ready-to-use. It can be stored at 4°C up to 6 month. For prolonged storage freeze the buffer in convenient aliquots at -20°C. Avoid repeated freezing and thawing! • Extraction Reagent The Extraction Reagent is supplied as dry powder and can be stored unopened at 4°C. The Extraction Reagent should be reconstituted just prior to use as described below. After reconstitution, it can be stored at 4°C up to 6 month. For prolonged storage freeze the buffers in convenient aliquots at -20°C. Avoid repeated freezing and thawing! • Reducing Agent The Reducing Agent is supplied as dry powder. It can be stored unopened at 4°C. The Reducing Agent should be reconstituted just prior to use as described below. After reconstitution, aliquot the unused material in 60 µl volumes and freeze at -20°C for further use. Avoid repeated freezing and thawing! • Benzonase® Benzonase® should be stored at 4°C. |
| Avoid freeze/thaw | Avoid freeze/thaw |
| Do not freeze | Ok to freeze |
| Packaging Information |
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| Transport Information |
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| Supplemental Information | |
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| Kit contains | Wash Buffer, Resuspension Buffer, Extraction Reagent, Reducing Reagent, Benzonase® Nuclease (Cat. No. 71206), Glass Beads, and a user protocol. |
| Specifications |
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