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| Description | |
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| Overview | The ProteoExtract® Subcellular Proteome Extraction Kit, Mini is a tool for the differential extraction of proteins from mammalian cells according to their subcellular localization. The sequential extraction steps yield proteins in their native state, making it highly suited for demanding proteomics applications. Sample size: 3-5x106 or 25-50 mg tissue. |
| Catalogue Number | 539791 |
| Brand Family | Calbiochem® |
| Synonyms | S-PEK Kit, Mini |
| Materials Required but Not Delivered | • Platform mixer (e.g. IKA Vibramax; for extracting adherent tissue culture cells) • Cell culture equipment, medium, and other reagents required for cell culture • Pipettors and tips for 10 µl, 200 µl, and 1 ml • Cooled centrifuge and rotor for 50 ml tube size • Cooled microcentrifuge and rotor up to 10,000 x g for 2 ml tube size • Thermo mixer or rolling facility |
| References | |
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| References | Yuan, X., et al. 2002. Electrophoresis 23, 1185. Butcher, B.A., et al. 2001. J. Immunol. 167, 2193; Ott, V., et al. 2001. Pharmacogenomics J. 1, 142. Allen, L., et al. 2000. Nature 405, 819. Rabilloud, T. 2000. Anal. Chem. 72, 48A. Mejdoubi, M., et al. 1999. Biochem. Biophys. Res. Commun. 254, 93. Reymond, et al. 1997. Electrophoresis 18, 2842. Laemmli, U. K. 1970. Nature 227, 680. Lowry, O.H., et al. 1951. J. Biol. Chem. 193, 265. |
| Product Information | |
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| Form | 10 Extractions |
| Kit contains | Wash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol. |
| Applications |
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| Biological Information | |
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| Sample Type | Mammalian tissues and cultured cells |
| Physicochemical Information |
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| Dimensions |
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| Toxicological Information |
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| Safety Information according to GHS |
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| Safety Information |
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| Product Usage Statements | |
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| Intended use | The Calbiochem® ProteoExtract® Subcellular Proteome Extraction Kit Mini (S-PEK) is intended for the differential extraction of proteins from mammalian cells according to their subcellular localization. The procedure yields proteins in their native state making it highly suited for demanding proteomics applications including enzyme activity assays (e.g. reporter gene assays) and subcellular redistribution assays to monitor protein shuttling (e.g. signaling proteins). |
| Storage and Shipping Information | |
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| Ship Code | Ambient Temperature Only |
| Toxicity | Multiple Toxicity Values, refer to MSDS |
| Storage | +2°C to +8°C |
| Storage Conditions | Upon arrival store the components of the kit under the following conditions: • Wash Buffer: Store at 4°C. • Extraction Buffers I-III: Store at 4°C for up to 6 months. For long-term storage, freeze the buffers in convenient aliquots at -20°C. • Extraction Buffer IV: Store at room temperature. • Protease Inhibitor Cocktail: The cocktail is supplied in DMSO and can be stored at 4°C for up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C. • Benzonase® Nuclease: Store at 4°C. |
| Do not freeze | Ok to freeze |
| Packaging Information |
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| Transport Information |
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| Supplemental Information | |
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| Kit contains | Wash buffer, Extraction Buffer I, Extraction Buffer II, Extraction Buffer III, Extraction Buffer IV, Protease Inhibitor Cocktail (Cat. No. 539134), Benzonase® Nuclease (Cat. No. 71206), and a user protocol. |
| Specifications |
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