详细说明
| Replacement Information |
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重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| ATR (Total), Chk1 (Ser345), Chk2 (Thr68), H2A.X (Ser139), MDM2 (total), p21 (Total), p53 (Ser15) | H, M, R | Mplex | Luminex xMAP |
| Description | |
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| Catalogue Number | 48-621MAG |
| Trade Name |
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| Description | MILLIPLEX MAP DNA Damage/Genotoxicity Magnetic Bead Panel - Cell Signaling Multiplex Assay |
| Overview | The MILLIPLEX® MAP 7-plex DNA Damage/Genotoxicity Magnetic Bead Kit (Cat. No. 48-621MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates: • ATR (total) • Chk1 (Ser345) • Chk2 (Thr68) • H2A.X (Ser139) • MDM2 (total) • p21 (total) • p53 (Ser15) |
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| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Panel Type | Cell Signaling |
| Applications | |
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| Application | Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting. |
| Key Applications |
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| Application Notes | • An overnight (4°C) incubation is recommended for best results. • This assay requires 25 µL diluted cell lysate per well. • This kit must be run using Assay Buffer 1 (provided). • The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 μL/well at 40 to 1,000 µg/mL). |
| Biological Information | |
|---|---|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
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| Assay Panel | Everything you need in a single kit. |
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| Storage Conditions | Recommended storage for kit components is 2 - 8°C. |
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| Material Size | 96-well plate |
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