详细说明
| Replacement Information |
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重要规格表
| Analytes Available | Species Reactivity | Key Applications | Detection Methods |
|---|---|---|---|
| JNK (Total), JNK (Thr183/Tyr185) | H, M, R | Mplex | Luminex xMAP |
| Description | |
|---|---|
| Catalogue Number | 48-622MAG |
| Trade Name |
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| Description | MILLIPLEX MAP 2-Plex Phospho/Total JNK Magnetic Bead Kit - Cell Signaling Multiplex Assay |
| Overview | The MILLIPLEX® MAP Total/Phospho Akt (Catalog #48-622MAG) has been developed for the simultaneous detection of phosphorylated JNK (Thr183/Tyr185) and total JNK in a single well using the Luminex system. |
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| Product Information | |
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| Detection method | Luminex xMAP |
| Configuration | Premixed |
| Panel Type | Cell Signaling |
| Applications | |
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| Application | Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. The analytes available for this multiplex kit are: Erk (Total), Erk (Thr185/Tyr187). |
| Key Applications |
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| Application Notes | o This is an overnight (4°C) incubation assay. o This kit must be run using the provided Assay Buffer. o This assay requires 25 μL diluted filtered cell lysate per well. o The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL). |
| Biological Information | |
|---|---|
| Specificity | Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible. |
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| Material Size | 96-well plate |
| Material Package | 96-well plate |
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