描述

<meta itemprop="description" content="Neuronal Isolation Enzyme (with papain) is a tissue-specific dissociation reagent for the isolation of primary cortical and hippocampal neurons from mouse/rat embryonic brain tissue and is a component of the Thermo Scientific Pierce Primary Neuron Isolation Kit (Product No. 88280). Papain has been " /> Neuronal Isolation Enzyme (with papain) is a tissue-specific dissociation reagent for the isolation of primary cortical and hippocampal neurons from mouse/rat embryonic brain tissue and is a component of the Thermo Scientific™ Pierce™ Primary Neuron Isolation Kit (Product No. 88280).

Papain has been demonstrated to be more effective and less harsh than other proteases for tissue dissociation. When used with Cardiomyocyte Isolation Enzyme 2 (with thermolysin), it improves digestion efficiency and cell yield of primary cardiomyocytes.

Papain Information:

• Source:Papaya latex
• Molecular mass: 23kDa
• Optimal pH: 6.0-7.0

More Product Data
• An efficient method for the isolation of highly functional primary neurons

Related Products
Pierce™ Primary Neuron Isolation Kit
Neuronal Culture Medium for Pierce™ Primary Cell Isolation Kits
Neuronal Culture Media Supplement (50X) for Pierce™ Primary Cell Isolation Kits
N-PER™ Neuronal Protein Extraction Reagent

For Research Use Only. Not for use in diagnostic procedures.

Figures

Primary cortical neuron cultures at different developmental stages in culture

Cell yield and viability after cell isolation using the Neuron Isolation Kit

规格

内容及储存

Stability: 1 year at -20°C  
Storage of Reconstituted Enzyme: -20°C for 6 months and up to two freeze-thaw cycles, or 1 week at 4°C. Enzyme use after prolonged storage may result in poor performance.