别名:   MGDF c-MPL Ligand

产品名称

Biochem/physiol Actions

TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. Recombinant murine TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.5-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Physical form

Lyophilized from 10 mM Tris, pH 8.0.

General description

TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity.

性质

recombinant	expressed in E. coli
assay	≥98% (HPLC)
	≥98% (SDS-PAGE)
form	lyophilized
mol wt	mol wt 18.7 kDa
impurities	<0.1 EU/μg endotoxin, tested
UniProt accession no.	P40226
shipped in	wet ice
storage temp.	?20°C
Gene Information	mouse ... Thpo(21832)