别名:   HGFR Hepatocyte growth factor receptor RCCP2 Scatter factor receptor met Proto-oncogene

产品名称

Application

Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

General description

Human cMet (GenBank Accession No. X54559), (amino acids 956-1390) with N-terminal GST tag, MW = 74 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 3 mM DTT and 20% glycerol.

性质

recombinant	expressed in baculovirus infected insect cells
assay	≥60% (SDS-PAGE)
form	aqueous solution
mol wt	mol wt 74 kDa
NCBI accession no.	X54559
shipped in	dry ice
storage temp.	?70°C
Gene Information	human ... MET(4233)