别名:   3-Phospho--glycerate carboxy-lyase(dimerizing) Rubisco

•  Enzyme Commission (EC) Number  4.1.1.39  ( BRENDA | IUBMB )

•  MDL number  MFCD00130908

• Popular Documents:  Specification Sheet (PDF)

产品名称

包装

1 unit in poly bottle

5 units in poly bottle

Unit Definition

One unit will convert 1.0 μmole of-RuDP and CO₂ to 2.0 μmoles of-3-phosphoglycerate per min at pH 7.8 at 25°C.

Application

D-Ribulose 1,5-diphosphate carboxylase from spinach has been used in a study to assess catalytic by-product formation and ligand binding by ribulose bisphosphate carboxylases from different phylogenies. D-Ribulose 1,5-diphosphate carboxylase from spinach has also been used in a study to determine RuBisCO activation kinetics and other rate and equilibrium constants by simultaneous multiple non-linear regression of a kinetic model.

General description

Exists as a 557 kDa hexadecamer composed of eight heavy chains each with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion.
pH optimum: ~7.9.
K_M for CO₂: ~0.45 mM.
Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM. Orthophosphate and ammonium sulfate are competitive inhibitors. 3-Phosphoglycerate is a noncompetitive inhibitor.

性质