详细说明
Enzyme Commission (EC) Number 5.1.3.1 ( BRENDA | IUBMB )
MDL number MFCD00130909
产品名称
Unit Definition
在 pH 7.7 和 25°C 下,与转酮醇酶、α-甘油磷酸脱氢酶和磷酸丙糖异构酶偶联时,一单位每分钟可将 1μmol 的-核酮糖-5-磷酸转化为-木酮糖-5-磷酸。
Physical form
基本上无硫酸盐的冻干粉:约含35% 的柠檬酸缓冲盐
Application
D-Ribulose-5-phosphate 3-Epimerase is an enzyme that converts the reversible conversion of D-ribulose 5-phosphate into D-xylulose 5-phosphate, which is important for the cellular response against oxidative stress . D-Ribulose-5-phosphate 3-Epimerase is involved in the pentose phosphate pathway, pentose and glucuronate interconversions and carbon fixation. Product R3251 is from baker’s yeast and is provided as a lyophilized powder. It is useful in enzyme systems requiring low sulfate.
Biochem/physiol Actions
RPE is a metalloenzyme and has been shown to use the divalent Zn2+ ion predominantly for catalysis. Human D-ribulose-5-phosphate 3-epimerase (hRPE) has been shown to use Fe2+ for catalysis .
性质
form | lyophilized powder |
foreign activity | phosphoriboisomerase, alcohol dehydrogenase, transketolase, and transaldolase<0.1% |
storage temp. | ?20°C |