别名:   Linoleate:oxygen oxidoreductase Lipoxygenase

•  EINECS 编号  232-853-1

•  Enzyme Commission (EC) Number  1.13.11.12  ( BRENDA | IUBMB )

•  MDL number  MFCD00131527

产品名称

Analysis Note

Protein determined by biuret.

Application

The soybean enzyme will use arachidonic acid as a substrate, with ~ 15% of the activity indicated using linoleic acid as the substrate; the product of arachidonic acid oxidation is 12- or 15-hydroperoxyarachidonic acid (12-HPETE or 15-HPETE).

Lipoxidase from Glycine max (soybean) has been used for the modification of low density lipoprotein, isolated from human plasma.

Biochem/physiol Actions

Catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure.

Preparation Note

Prepared by ion exchange chromatography and hydrophobic interaction chromatography.

Unit Definition

One unit will cause an increase in A₂₃₄ of 0.001 per min at pH 9.0 at 25 °C when linoleic acid is the substrate in 3.0 ml volume (1 cm light path). One A₂₃₄ unit is equivalent to the oxidation of 0.12 μmole of linoleic acid.

Physical form

Suspension in 2.3 M (NH₄)₂SO₄ solution, pH approx. 6.0

性质