详细说明
描述
<meta itemprop="description" content="The ImaGene Red lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C12RG. Once inside the cell, the substrates are cleaved by -galactosidase, producing a fluorescent product that is well retained b" /> The ImaGene Red™ lacZ Gene Expression Kit contains a fluorescein-based galactosidase substrate that has been covalently modified to include a 12-carbon lipophilic moiety, C 12RG. Once inside the cell, the substrates are cleaved by β-galactosidase, producing a fluorescent product that is well retained by the cells, probably by incorporation of the lipophilic tail within the cellular membrane. In addition to the C 12RG, the kits also include the broad-spectrum β-galactosidase inhibitor, PETG, and chloroquine diphosphate for inhibiting acidic hydrolysis of the substrate.
规格
Assay Type: | β-Gal (lacZ) Assay |
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Detection Method: | Fluorescent |
Excitation⁄Emission (nm): | 571⁄585 |
Product Line: | ImaGene Green™ |
Product Size: | 1 kit |
Substrate: | C12RG |
Substrate Properties: | Chemical Substrate |
Substrate Type: | Beta-Gal Substrate |
Target Enzyme: | Beta-Galactosidase |
Shipping Condition: | Room Temperature |
内容及储存
Store in freezer (-5 to -30°C) and protect from light.