质量水平

100

生物来源

human dermis (fibroblast)

重编程方法

episomal

描述

age (15-19)

manufacturer/tradename

EBiSC

性别

male

生长模式

adherent (pluripotent)

应用

cell culture | stem cell: suitable

相关疾病

Alzheimer′s disease

运输

dry ice

储存温度

−196°C

相关类别

Stem Cells

一般描述

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells.  Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from  neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

细胞系来源

Depositor
Bioneer

细胞系描述

Derivation
Primary cell type: Fibroblast of dermis
Primary cell developmental stage: Adult
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
shP53
SOX2
Other: from the same donor: BIONi010-A, BIONi010-B

Characterization
Differentiation potency
Ectoderm: HES5 (+),NeuroD1 (+),PAX6 (+),Endoderm: CXCR4  (+),GSC (+),PITX1 (+),Mesoderm: DCN(+),NCAM1 (+),VIM(+)

Microbiology / Virus Screening
HIV 1:Negative
HIV 2:Negative
Hepatitis B:Negative
Hepatitis C: Negative
Mycoplasma:Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 31
Cell line karyotype: 46 XY
Karyotyping method: G-Banding
Genotyping
STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimers disease
Type of modification: Gene knock-out
Gene: APOE
Chromosome location: 19q13.32
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: The ApoE gene is knocked out in this line; deletion of the ApoE gene by insertion of a frame shift in exon 1. The target locus has been mutated.

联系

Additional Cell Line Info

• EBiSC Access Use Agreement (EAUA)

• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

传代培养常规

Medium: -
Passage method: EDTA
Matrix: Matrigel^® / Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 18%
Temperature: 37°C

法律信息

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.