生物来源

human dermis (fibroblast)

重编程方法

mRNA

描述

age (25-29)

manufacturer/tradename

EBiSC

性别

female

生长模式

adherent (pluripotent)

应用

cell culture | stem cell: suitable

相关疾病

muscular dystrophy (myotonic type 1)

运输

dry ice

储存温度

−196°C

相关类别

Stem Cells

一般描述

Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells.  Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.

The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from  neurodegenerative diseases (Alzheimers Disease, Parkinsons Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.

细胞系来源

Depositor
Censo Biotechnologies

细胞系描述

Derivation
Primary cell type: Fibroblast
Tissue collection year: 2015
Reprogramming method
Vector type: None
Vector Free Reprogramming
Types of factor(s) used: mRNA
mRNA: NANOG
LIN28
MYC
KLF4
SOX2
POU5F1

Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no

Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
SSEA-1 No
POU5F1 (OCT-4) Yes
TRA 1-60 Yes
SSEA-4 Yes
Differentiation potency
Ectoderm:
Ectoderm
In vitro spontaneous differentiation
Marker Expressed
HES5 Yes
NeuroD1 Yes
PAX6 Yes
Endoderm:
Endoderm
In vitro spontaneous differentiation
Marker Expressed
GATA6 Yes
MIXL1 Yes
Mesoderm:
Mesoderm
In vitro spontaneous differentiation
Marker Expressed
NCAM1 Yes
VIM Yes
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Sterility
Inoculation for microbiological growth: No Contaminants Detected
Mycoplasma: Not Detected
Viability: Viable post-cryopreservation

Genotyping
Karyotyping
Passage number: 10
Cell line karyotype: No autosomal or sex aneuploidies were detected in this sample
Karyotyping method: KaryoLite BoBs

STR/Fingerprinting: A 16 allele profile has been recorded and data is available upon request, after cell line purchase.

联系

Additional Cell Line Info

• EBiSC Access Use Agreement (EAUA)

• Cell Line Information Pack (CLIP)

Note: EAUA and CLIP must be completed before order fulfillment

传代培养常规

Medium: mTeSR^®
Passage method: EDTA
Matrix: Matrigel^® / Geltrex^®
CO₂ concentration: 5%
O₂ concentration: 21%
Temperature: 37°C

法律信息

GELTREX is a registered trademark of Life Technologies Corporation

Matrigel is a registered trademark of Corning, Inc.

mTeSR is a registered trademark of WiCell Research Institute, Inc.