生物来源

human skin

描述

Human melanoma, metastasis

生长模式

Adherent

形态学

Epithelial

应用

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

相关类别

Mammalian Cell Lines

细胞系描述

Ma-Mel-19 (ESTDAB-152) was established from the cutaneous/subcutaneous metastasis in a 62 year-old female patient with metastatic melanoma (superficial spreading melanoma stage IV). The Ma-Mel-19 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission′s fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

DNA图谱分析

STR-PCR Data:

Amelogenin: X
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11,13
D5S818: 9,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 14,18

培养基

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) seed new cultures at 1-3 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 8 x10,000 cells/cm² .

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.