生物来源

mouse blood

生长模式

Suspension

形态学

Lymphoblast

相关疾病

cancer

运输

dry ice

储存温度

−196°C

相关类别

Mammalian Cell Lines

一般描述

For complete product information, please see BW5147.G.1.4.OUA/R.1

细胞系来源

Mouse AKR/J T cell lymphoma

细胞系描述

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

应用

Fusion partner

培养基

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 1-6x100,000 cells/ml; 5% CO₂; 37°C.

其他说明

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