生物来源

human ovary

生长模式

Adherent

核型

For modal chromosome numbers see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.

形态学

Epithelial; stellate in shape and producing hemicystic colonies which grow upwards from the substrate and detach as spheres.

产品

Not specified

受体

Oestrogen receptor negative cell line. For antigen expression see Langdon SP et.al. (1988) Cancer Research Nov 1; 48(21):6166-72.

应用

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

相关类别

Mammalian Cell Lines

细胞系来源

Human ovarian cancer; oestrogen receptor negative

DNA图谱分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 11,12
D7S820: 9,10
THO1: 6
TPOX: 8,11
vWA: 15,16

培养基

RPMI 1640 + 2 mM Glutamine + 2 mM Sodium Pyruvate + 10% Fetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2- 3 x 10⁴ cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.  Doubling time approximately 120 hours.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.