生物来源

human colon

生长模式

Adherent

核型

Not specified

形态学

Epithelial

产品

Not specified

受体

Not specified

应用

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

相关类别

Mammalian Cell Lines

细胞系来源

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage C

细胞系描述

The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes′ stage C.

DNA图谱分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,14
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,10
vWA: 16,17

培养基

Iscove′s Modified Dulbecco′s Medium, + 10%  Foetal Bovine Serum (FBS) + 2 mM Glutamine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. C99 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.