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产品描述:
Keratinocye Growth Factor-2 (KGF-2), also known as FGF-10, was originally identified from rat embryos by homology-based polymerase chain reaction. Human and mouse KGF-2 were subsequently cloned. The human KGF-2 cDNA encodes a 208 amino acid residue protein with a hydrophobic amino-terminal signal peptide. Human KGF-2 shares approximately 92% and 95% amino acid sequence identity with mouse and rat KGF-2, respectively. Among the FGF family members, KGF-2 is most closely related to FGF-7. The expression of KGF-2 transcripts has been shown to be most abundant in the embryo and adult lung. Recombinant KGF-2 preparations have been shown to be mitogenic for epithelial and epidermal cells but not fibroblasts. Based on its in vitro biological activities and in vivo expression pattern, KGF-2 has been proposed to play unique roles in the brain, in lung development, wound healing and limb bud formation.

表达系统 :
Escherichia coli

溶解方法:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20°C. Further dilutions should be made in appropriate buffered solutions.

产品组成:
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.

存储条件:
This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

分子量:
Approximately 19.3 kDa, 169 amino acid residues consisting of the mature human KGF-2 (amino acid residues 40 – 208).

内毒素:
Less than 1 EU/μg of KGF-2/FGF-10 as determined by LAL method.

产品用途:
This material is offered by Cyagen Biosciences for research, laboratory or further evaluation purposes. FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAP EUTIC OR DIAGNOSTIC USE.

生物学活性:
The ED50 determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 100 ng/mL, corresponding to a specific activity of > 1.0 × 10⁴ IU/mg.

产品形态:
Sterile filtered white lyophilized (freeze-dried) powder.

氨基酸序列:
LGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS

产品纯度:
> 97% by SDS-PAGE and HPLC analyses.