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TAMRA Thiol is an inactive form of bright yellow dye that used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 553/575 nm, similar to that of DyLight 549, ATTO 550 and Cy 3. TAMRA might be excited using 543 or 546 nm laser line and displays good optical property. The thiol is attached to TAMRA fluorophore through an aminoethyl linkage. TAMRA thiol can be labeled to biomolecules through a disulfide bond formation with thiol of cysteine residue or can be utilized as a reference standard for dye-conjugates.
Fluorophore: TAMRA
Functional group: Thiol
Excitation/Emission Max.(nm): 553/575
Spectrally similar dyes: DyLight549, Cy3, ATTO550
Extinction coefficient: ≥ 70,000 cm-1M-1
CF280: 0.21
Appearance: Red Solid
Molecular Weight: 491.60 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light