生物来源

human blood

种族

Caucasian

性别

male

生长模式

Suspension

核型

Not specified

形态学

Lymphoblastoid

应用

cell culture | mammalian: suitable

运输

dry ice

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相关类别

Mammalian Cell Lines

细胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC).  HLA-Typing Data is available for this cell line.

DNA图谱分析

Not specified

血清学分析

HLA A: 1 3
HLA C: 7 0
HLA B: 8 0
HLA DR: 3 0
HLA DQ: 0 2

培养基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442).  Once a growing culture is established the FBS concentration can be reduced to 10%.

传代培养常规

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C.  Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

免责声明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.