质量水平

100

生物来源

human neonatal foreskin or adult skin (normal)

包装

pkg of 500,000 cells

生长模式

Adherent

核型

2n = 46

形态学

Fibroblast

应用

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

相关类别

Primary Cells

一般描述

Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)

细胞系来源

Skin

应用

injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response

组分

Fibroblast Basal Medium containing 10% FBS & 10% DMSO

制备说明

• Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO

• Can be cultured at least 16 doublings

传代培养常规

Please refer to the HDF Culture Protocol.