生物来源

rat dermis

质量水平

100

包装

pkg of 500,000 cells

生长模式

Adherent

形态学

Fibroblast

应用

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

相关类别

Primary Cells

一般描述

RDF from Cell Applications, Inc. have been used in allogenic prosthetic grafts, demonstrating improved epithelization, collagen organization and faster regeneration (Nomoto, 2012), in a cell sheet engineering study, where a sheet of dermal fibroblasts was transplanted into the damaged liver in allogeneic rat model to promote migration of hepatic progenitor cells and faster healing (Muraoka, 2013), and as a control in engineered cardiac tissues (Serrao, 2012).

细胞系来源

Skin

应用

Injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response.

组分

Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

质量

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

制备说明

• 1st passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO

• Can be cultured at least 8 population doublings

传代培养常规

Please refer to the RDF Culture Protocol.