生物来源

rat skin (Sprague Dawley)

质量水平

100

包装

pkg of 500,000 cells

生长模式

Adherent

形态学

Epidermal

应用

cell culture | mammalian: suitable

相关疾病

allergies; cancer

运输

dry ice

储存温度

−196°C

相关类别

Primary Cells

一般描述

REK from Cell Applications, Inc. provide a suitable model system to study many aspects of epithelial function and disease, particularly those related to skin biology and toxicology.  It was shown that TGFβ1 treatment decreased c-Myc mRNA in REK which, taken together with the TGFβ1-induced growth arrest exhibited by keratinocytes and opposite results obtained in other cells, suggests a correlation between c-Myc mRNA expression and the mitogenic response (Nakai, 2008). Additionally, REK were used to demonstrate that pathological increases in keratinocyte sodium channels Nav1.1, Nav1.6, and Nav1.8 expression may contribute to pain by increasing epidermal ATP release, resulting in excessive activation of P2X receptors on primary sensory axons (Zhao, 2008). REK were also used to demonstrate that laminin 332 deposition is inhibited by ionizing radiation and, in combination with slower keratinocyte migration, can contribute to the delayed wound healing of irradiated skin (Jourdan, 2011).

细胞系来源

Skin

应用

Skin biology and toxicology, wound healing, artificial skin, cell proliferation, migration, UV light response.

组分

Rat Keratinocyte Basal Medium that contains 10% FBS and 10% DMSO

质量

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

制备说明

• Primary culture, >500,000 cells in Rat Keratinocyte Basal Medium that contains 10% FBS and 10% DMSO

• These epithelial cells proliferate well when thawed and plated from cryopreservation, but they do not plate and proliferate after trypsinization

传代培养常规

Please refer to the RDF Culture Protocol.