Formula variant

如 Murashige 和 Skoog (1962) 所述含常量营养素和微量营养素。

Preparation Note

Murashige Skoog medium can be reconstituted from powder by combining products that are major components of complete M&S medium, such as macronutrient mixtures vitamin mixtures. Murashige Skoog Salt mixture (M5524) contains the macronutrients micronutrients of the original classic formulation. It can be combined with M&S vitamins Gamborg’s vitamins supplemented with sucrose, agar, auxins (IAA) cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.

Quantity

配制成每升培养基含 4.3 克粉末。

Application

Murashige Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate organic additives such as agar, sugars, vitamins growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) the Kinetin (cytokinin/cell division promoter).

Other Notes

Storage
Powdered media are extremely hygroscopic must be protected from atmospheric moisture. If possible the entire contents of each package should be used immediately after opening.

Store dry medium in a desiccator at 0-5 °C.

Deterioration of powdered medium may be recognized by: 1) color change; 2) granulation, clumping, particulate matter throughout the powder; 3) insolubility; 4) pH change; 5) inability to promote growth when properly used.

Media Preparation