描述

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

The complete formulation is available.

Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12  PABA which are not found in Eagle's Minimal Essential Medium Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

cGMP Manufacturing Quality System
Gibco® RPMI 1640 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer is certified to ISO 13485 ISO 9001 standards. For supply chain continuity, Life Technologies offers a comparable Gibco® RPMI 1640 product made in our Scotland facility (51800-019). This facility is registered with the FDA as a medical device manufacturer is certified to the ISO 13485 standard.

RPMI 1640 contains no proteins, lipids, growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g / L) therefore requires a 5-10% CO2 environment to maintain physiological pH.

Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, filtration at the time of preparation (see protocol for details).

在中国境内, 本产品仅限于非临床科研用途, 产品信息以中文为准。 (In China territory, the product is for Research Use only in non-clinical unit, product information in Chinese shall prevail.)

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规格

培养基配方

31800 - RPMI 1640, powder