应用；   For immunoprecipitation of recombinant HA-tagged proteins  

* Refer to Certificate of Analysis for lot specific data (including water content).

介绍 技术信息 安全信息 安全技术说明书和分析证明书

Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells or in vitro systems, using manual or robotic magnetic separators.

Features of Anti-HA Magnetic Beads:
• Specific—highly specific anti-HA monoclonal antibody (clone 2-2.2.14) enables high yield and high purity immunoprecipitation.
• Convenient and fast—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately one hour.
• Low non-specific binding—stable, pre-blocked beads and specific antibody minimize off-target binding for HA-tag IP or co-IP experiments.
• Versatile—beads are compatible with manual and automated workflows.

The blocked magnetic bead surface is coated with anti-HA antibody, a highly specific mouse IgG1 monoclonal antibody that recognizes the HA-epitope tag (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. The Anti-HA Magnetic Beads can be used manually with a magnetic stand, as well as with automated platforms.

Product Details:
Anti-HA Magnetic Beads are convenient for the immunoprecipitation (IP) of recombinant HA-tagged proteins and the co-immunoprecipitation (co-IP) of their interacting proteins. The beads are incubated with a cell lysate containing HA-tagged protein and the fusion protein is captured. The beads are subsequently washed and then the target proteins are eluted using 0.1M glycine (pH 2.0), 50 mM NaOH, or SDS-PAGE sample buffer. If gentler elution conditions are desired, 2 mg/mL of HA peptide can be used.

用法 :

•Do not centrifuge, dry or freeze Anti-HA Magnetic Beads. Centrifuging, drying or freezing will cause the beads to aggregate and lose binding activity. To ensure good dispersal of beads for optimal antibody binding, it is important to include 0.025% to 0.1% non-ionic (e.g.,     sc-29113, Tween-20 Detergent) or zwitterionic (e.g.,     sc-29088, CHAPS) detergent in the binding and wash buffers and to mix the beads during incubation.    
• For best results, determine optimal conditions for expression of HA-tagged fusion protein before attempting immunoprecipitation.    
• To minimize protein degradation, include protease inhibitors (such as:     sc-29130, UltraCruz® Protease Inhibitor Cocktail Tablet or     sc-29131, UltraCruz® Protease Inhibitor Cocktail Tablet, EDTA-free) in the preparation of cell lysates.    
• Binding capacity and elution recovery will vary depending on the HA-fusion protein and the method of elution.    
• A low-pH elution may be used for single-use applications. Optimal incubation time for low-pH elution is 5-10 minutes; exceeding 10 minutes may result in nonspecific binding and yield reduction. The HA antibody will not leach from the beads when eluting with the recommended acidic elution buffer (0.1M glycine, pH 2.0).    
• Basic elution buffer (e.g., 50mM NaOH) may be used to elute HA-tagged protein; however, the stringency of the buffer will cause the HA antibody to leach from the beads.    
• If a gentle elution of HA-tagged protein is desired, a competitive elution can be performed using 2mg/mL HA Peptide.    
• Anti-HA Magnetic Beads are compatible with immunoprecipitation and analyses by Western blot.    
• Do not use cell lysate containing dithiothreitol (DTT). DTT may cause the HA antibody to leach from the beads.

外观 :

Suspension

物理状态 :

Liquid

保存 :

Store at 4° C

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.