详细说明
Product Brief
Introduction
Goat Anti-mouse IgG（H+L） secondary antibody, 5 nm Colloidal Gold Conjugate contains affinity-purified antibodies with well-characterized specificity for Mouse IgG (whole molecule) and are useful in the detection of their specified targets through IHC-P,IHC-F,ICC,EM.
Overview
Product Name 5nm Colloidal Gold Labeled Secondary Antibody, Goat Anti-Mouse IgG（H+L） SKU/Catalog Number GA1003 Description This antibody is purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG. The antibody preparation is solid phase adsorbed with human serum proteins to ensure minimal cross reactivity in tissue or cell preparations. The goat anti-mouse IgG is conjugated to 5 nm colloidal gold and generates electron dense particles that are visible using electron microscopy. Cite This Product 5nm Colloidal Gold Labeled Secondary Antibody, Goat Anti-Mouse IgG (Boster Biological Technology, Wuhan, CHINA, Catalog # GA1003) Host Goat Validated Species Mouse Application EM, IHC, ICC Immunogen Mouse IgG (whole molecule). Conjugate 5 nm Colloidal Gold Pack Size 0.5ml/1ml Properties
Clonality Polyclonal Storage At 4°C for one year. Background
EM-grade colloidal gold conjugated secondary antibodies are useful for the applications of light microscopy and immunoblotting, as well as electron microscopy. Monomeric gold-protein conjugates are separated from aggregates by ultracentrifugation and careful separation by density gradients, resulting in consistent and reproducible gold-IgG conjugates. Boster’s colloidal gold conjugated secondary antibodies are guaranteed to have high affinity and low cross-reactivity.
Instructions
胶体金稀释液：PBS 或 TBS 缓冲液，加入试剂级 BSA 至 1%。 使用上述胶体金稀释液，免疫电镜按 1：20—50 稀释，免疫组化按 1：50—100 稀释。胶体金 稀释后溶液应于当天使用，未用完的应弃置。
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