概述

• 产品名称Anti-alpha Actinin抗体[EP2527Y]
     

• 描述

  兔单克隆抗体[EP2527Y] to alpha Actinin

• 经测试应用WB,IP,Flow Cyt,IHC-P

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  A synthetic peptide corresponding to residues on the N-terminus of human alpha Actinin

• 阳性对照    

• NIH 3T3 lysate, HeLa lysate and C6 lysate

• 常规说明

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

• 存储溶液pH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA

• 纯度Tissue culture supernatant

• 克隆单克隆

• 克隆编号EP2527Y

• 同种型IgG

• 研究领域

• Developmental Biology

• Lineage specification

• Mesoderm

• Stem Cells

• Lineage Markers

• Mesoderm

• Signal Transduction

• Cytoskeleton / ECM

• Cytoskeleton

• Microfilaments

• Actin etc

• Actin Binding Proteins

• <a javascript:void(0)>Signal Transduction

• Cytoskeleton / ECM

• Cytoskeleton

• Microfilaments

• Actin etc

• Actin Crosslinking

Anti-alpha Actinin antibody [EP2527Y] 图像

• 

  Flow Cytometry - Anti-alpha Actinin antibody [EP2527Y] (ab68194)          

     

  Overlay histogram showing HeLa cells stained with ab68194 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab68194, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

   

• 

  Western blot - alpha Actinin antibody [EP2527Y] (ab68194)          

     All lanes : Anti-alpha Actinin antibody [EP2527Y] (ab68194) at 1/10000 dilution
  
  Lane 1 : NIH 3T3 lysate
  Lane 2 : HeLa lysate
  Lane 3 : C6 lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 103 kDa
  Observed band size : 103 kDa
   

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Actinin antibody [EP2527Y] (ab68194)      This image is courtesy of an Abreview submitted by Kadir Demircan    

     

  ab68194 staining alpha Actinin in rat kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 30% BSA for 10 minutes at 37°C; antigen retrieval was by heat mediation in EDTA. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. ab80436 EXPOSE Mouse and Rabbit Specific HRP/DAB Detection IHC kit was used.

     

  See Abreview