概述

• 产品名称Anti-alpha Actinin抗体[EP2528Y]
     

• 描述

  兔单克隆抗体[EP2528Y] to alpha Actinin

• 经测试应用WB

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human alpha Actinin aa 750-850 (C terminal). The exact sequence is proprietary.

• 阳性对照    

• WB: NIH 3T3, C2C12, C6 and A673 cell lysates and human skeletal muscle and human heart tissue lysates.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam's RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EP2528Y

• 同种型IgG

• 研究领域

• Developmental Biology

• Lineage specification

• Mesoderm

• Stem Cells

• Lineage Markers

• Mesoderm

• Signal Transduction

• Cytoskeleton / ECM

• Cytoskeleton

• Microfilaments

• Actin etc

• Actin Binding Proteins

• <a javascript:void(0)>Signal Transduction

• Cytoskeleton / ECM

• Cytoskeleton

• Microfilaments

• Actin etc

• Actin Crosslinking

Anti-alpha Actinin antibody [EP2528Y] 图像

• 

  Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)          

     All lanes : Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified)
  
  Lane 1 : NIH/3T3 whole cell lysate
  Lane 2 : C2C12 whole cell lysate
  Lane 3 : C6 whole cell lysate
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
  
  Predicted band size : 103 kDa
  Observed band size : 103 kDa
     Blocking and dilution buffer: 5% NFDM/TBST  

• 

  Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)          

     Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (purified) + A673 whole cell lysate at 20 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
  
  Predicted band size : 103 kDa
  Observed band size : 103 kDa
     Blocking and dilution buffer: 5% NFDM/TBST  

• 

  Western blot - Anti-alpha Actinin antibody [EP2528Y] (ab81265)          

     All lanes : Anti-alpha Actinin antibody [EP2528Y] (ab81265) at 1/1000 dilution (unpurified)
  
  Lane 1 : NIH 3T3 cell lysate
  Lane 2 : human skeletal muscle tissue lysate
  Lane 3 : human heart tissue lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
  
  Predicted band size : 103 kDa
  Observed band size : 103 kDa