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详细说明
概述
产品名称Anti-Amyloid Fibril抗体[mOC87] - Conformation-Specific
描述
兔单克隆抗体[mOC87] to Amyloid Fibril - Conformation-Specific
经测试应用Dot Blot,IHC-FoFr,IHC-P,IHC-FrFl
种属反应性
与反应: Mouse, Human
免疫原
corresponding to Human Amyloid Fibril. Amyloid beta 1-42 fibrils were used as the immunogen.
Database link: P05067阳性对照
beta Amyloid (Aß) 1-40; beta Amyloid (Aß) 1-42. IHC-P: FFPE human Alzheimer hippocampus tissue sections.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
This antibody was developed as part of a collaboration between Abcam and Professor Charles Glabe, UC Irvine.
ab201062 recognizes a generic epitope of amyloid fibrils and oligomers that is independent of linear sequence (Hatami et al. 2014). Its reactivity with Aß monomer and oligomers is decreased or eliminated upon thermal denaturation at 100°C of Aß in SDS sample buffer on western blots (Hatami et al. 2014).
For further information on the immunogen, please refer to Hatami et al. 2014 and Kayed et al. 2007.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号mOC87
同种型IgG
研究领域
Neuroscience
Neurology process
Neurogenesis
Neuroscience
Neurology process
Growth and Development
Axonal Guidance Proteins
Neuroscience
Neurology process
Neurodegenerative disease
Alzheimer's disease
Amyloid
Neuroscience
Cell Adhesion Proteins
Membrane Proteins
Neuroscience
Neurology process
Notch Pathway
Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific 图像
![Immunohistochemistry - Free Floating - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2010624X2.jpg)
Immunohistochemistry - Free Floating - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062) Image courtesy of Professor Charles Glabe, UC Irvine
Immunohistochemical staining of human brain tissue from a patient with a diagnosis of Alzheimers disease, male, 81 years, 5 hour post mortem index, tangle stage 5, plaque stage B, mini mental status exam score 12. Sections were cut using a vibratome. No antigen retrieval was performed. Free floating sections were stained using ab201062 at a dilution of 50 ng/mL. The secondary antibody used was a biotinylated goat anti-rabbit at a dilution of 1/225, which was blocked with normal goat serum. The sample was visualized using ABC solution (1 hour incubation) followed by 1-4 minutes of DAB. The sample was mounted and allowed to dry overnight, followed by dehydration in increasingly concentrated ethanol solutions.
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201062510.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062) Image is courtesy of an AbReview submitted by Dr Ruma Raha-Chowdhury.
Immunohistochemical analysis of 4% PFA in 0.1M PBS perfusion-fixed murine APP-PS1 transgenic brain tissue sections, labelling beta amyloid with ab201062 at a dilution of 1/200 incubated for 24 hours at 4°C in 0.1 M PBST with 10% donkey serum. Permeabilization was 0.1M PBS with 3% Triton X. Secondary was a polyclonal rabbit Alexa Fluor® 488 at 1/100. Counterstaining was DAPI against nuclear DNA and astrocytes stained with GFAP-Cy3.
See Abreview
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201062hip.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
IHC image of beta Amyloid staining in human Alzheimer hippocampus formalin fixed paraffin embedded tissue section*, performed on a Leica Bond? system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201062, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
![Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab20106262a.jpg)
Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
Dot blot analysis of beta Amyloid 1-42 labeled with ab201062 at 1/8000 dilution.
Lane 1: beta Amyloid (Aβ) 1-40.
Lane 2: beta Amyloid (Aβ) 1-42.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.Antibody reactivity was assessed using a dot blot, which is a non-quantitative method that maintains the native conformation of beta Amyloid. Beta Amyloid 1-40 and 1-42 peptides underwent the following aggregation conditions before being spotted onto a nitrocellulose membrane and detected using ab201062:
Monomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 1% SDS and boiled for five minutes.
Oligomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 10 mM phosphate buffer pH 7.4 containing 0.02% sodium azide and incubated at room temperature for four days.
Fibrils: 0.3 mg of beta Amyloid peptide was dissolved in 1 ml 50% hexafluoroisopropanol (HFIP) with 0.02% sodium azide. It was then stirred constantly for nine days; the first seven with a cap on and the final two with the cap removed to allow evaporation of the HFIP. Fibrils were then sedimented at 20,000 rpm in a microcentrifuge for 20 minutes and resuspended in 1 ml of PBS + 0.02% sodium azide.![Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab20106262b.jpg)
Dot Blot - Anti-Amyloid Fibril antibody [mOC87] - Conformation-Specific (ab201062)
Negative control (secondary ab only):
Lane 1: beta Amyloid (Aβ) 1-40.
Lane 2: beta Amyloid (Aβ) 1-42.
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 30 seconds.
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