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详细说明
概述
产品名称Anti-Amyloid Precursor Protein (phospho T743)抗体[EPR7074(N)]
描述
兔单克隆抗体[EPR7074(N)] to Amyloid Precursor Protein (phospho T743)
经测试应用WB,ICC/IF,Dot Blot,IP
种属反应性
与反应: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Amyloid Precursor Protein aa 700 to the C-terminus (phospho T743). The exact sequence is proprietary.
Database link: P05067阳性对照
WB: Human hippocampus lysate; HeLa treated with 40ng/ml nocodazole for 24 hours whole cell lysate; Mouse and rat brain lysates. ICC/IF: HeLa cells. IP: Mouse brain whole cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR7074(N)
同种型IgG
研究领域
Neuroscience
Neurology process
Neurodegenerative disease
Alzheimer's disease
Amyloid
Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] 图像
![Dot Blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2062977DB.jpg)
Dot Blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)
Dot blot analysis of Amyloid Precursor Protein (phospho T668) phospho peptide (Lane 1) and Amyloid Precursor Protein Non-phospho peptide (Lane 2) labeled using ab206297 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
![Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab206297WBa.jpg)
Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)
All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/5000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human hippocampus treated with Lambda Phosphatase lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size : 86 kDa
Observed band size : 100-120 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab206297WBb.jpg)
Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)
All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/5000 dilution
Lane 1 : Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 40ng/ml nocodazole for 24 hours whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size : 86 kDa
Observed band size : 100-120 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab206297WBc.jpg)
Western blot - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)
All lanes : Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 86 kDa
Observed band size : 100-120 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Immunocytochemistry/ Immunofluorescence - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2062977IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Amyloid Precursor Protein (phospho T668) with ab206297 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The expression increased after treatment with nocodazole (0.04ug/ml) for 24 hours. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows:-
-ve control 1: ab206297 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
![Immunoprecipitation - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2062977IP.jpg)
Immunoprecipitation - Anti-Amyloid Precursor Protein (phospho T743) antibody [EPR7074(N)] (ab206297)
Amyloid Precursor Protein (phospho T668) was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab206297 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab206297 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366), was used as secondary antibody at 1/10000 dilution.
Lane 1: Mouse brain whole cell lysate 10ug (Input). Lane 2: ab206297 IP in Mouse brain whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab206297 in Mouse brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
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