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详细说明
概述
产品名称Anti-AP3M1抗体[EPR16385]
描述
兔单克隆抗体[EPR16385] to AP3M1
经测试应用WB,ICC/IF,Flow Cyt
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human AP3M1 aa 100-200. The exact sequence is proprietary.
Database link: Q9Y2T2阳性对照
WB: HeLa and HEK293 cell lysates; Human fetal brain lysate. ICC/IF: HeLa cells Flow Cyt: HEK293 cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR16385
同种型IgG
研究领域
Signal Transduction
Protein Trafficking
Golgi Proteins
Signal Transduction
Protein Trafficking
Vesicle Transport
Coat Proteins
Anti-AP3M1 antibody [EPR16385] 图像
![Immunocytochemistry/ Immunofluorescence - Anti-AP3M1 antibody [EPR16385] (ab201227)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab201227227.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-AP3M1 antibody [EPR16385] (ab201227)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling AP3M1 with ab201227 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows:
-ve control 1: ab201227 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
-ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.![Western blot - Anti-AP3M1 antibody [EPR16385] (ab201227)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2012277WB.jpg)
Western blot - Anti-AP3M1 antibody [EPR16385] (ab201227)
All lanes : Anti-AP3M1 antibody [EPR16385] (ab201227) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
Lane 2 : HEK293 (Human embryonic kidney) cell lysate
Lane 3 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 47 kDa
Observed band size : 47 kDa
Exposure time : 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
![Flow Cytometry - Anti-AP3M1 antibody [EPR16385] (ab201227)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab2/ab2012277FC.jpg)
Flow Cytometry - Anti-AP3M1 antibody [EPR16385] (ab201227)
Flow cytometric analysis of 2% paraformaldehyde-fixed HEK293 (Human embryonic kidney) cells labeling AP3M1 with ab201227 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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