概述

• 产品名称Anti-Bad (phospho S112)抗体[EPR1891(2)]
     

• 描述

  兔单克隆抗体[EPR1891(2)] to Bad (phospho S112)

• 经测试应用WB,IP

• 种属反应性

  与反应: Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Bad.
  (Peptide available as

• 阳性对照    

• HeLa cell lysates treated with Calyculin A.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 解离常数（K_D） K_D = 4.70 x 10 ^-11 M Learn more about K_D

• 存储溶液pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR1891(2)

• 同种型IgG

• 研究领域

• Cancer

• Cell Death

• Apoptosis

• Metabolism

• Cancer

• Cell Death

• Apoptosis

• Apoptotic Markers

• Bcl 2 family

• Metabolism

• Pathways and Processes

• Metabolism processes

• Apoptosis

• Cancer

• Invasion/microenvironment

• Apoptosis

• Bcl 2 family

• Cell Biology

• Apoptosis

• Intracellular

• Bcl2 Family

Anti-Bad (phospho S112) antibody [EPR1891(2)] 图像

• 

  Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)          

     All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/5000 dilution (purified)
  
  Lane 1 : Untreated C6 cells
  Lane 2 : C6 cells treated with Calcyculin A
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP goat anti-rabbit (H+L) at 1/1000 dilution
  
  Predicted band size : 18 kDa
  Observed band size : 23 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer: 5% NFDM/TBST

  Dilution buffer: 5% NFDM/TBST

   

• 

  Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)          

     All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/20000 dilution (purified)
  
  Lane 1 : Untreated HeLa cells
  Lane 2 : HeLa cells treated with Calcyculin A
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP goat anti-rabbit (H+L) at 1/1000 dilution
  
  Predicted band size : 18 kDa
  Observed band size : 23 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer: 5% NFDM/TBST

  Dilution buffer: 5% NFDM/TBST

   

• 

  Immunoprecipitation - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)          

     

  ab129192 (purified) at 1/30 immunoprecipitating Bad in HeLa cell lysate treated with Calcyculin A (Lane 1). For western blotting a HRP-conjugated anti-rabbit IgG was used as the secondary antibody (1/1000).

  Blocking buffer and concentration: 5% NFDM/TBST.

  Diluting buffer and concentration: 5% NFDM /TBST.

   

• 

  Western blot - Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192)          

     All lanes : Anti-Bad (phospho S112) antibody [EPR1891(2)] (ab129192) at 1/1000 dilution
  
  Lane 1 : HeLa cell lysates, untreated
  Lane 2 : HeLa cell lysates treated with Calyculin A.
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 18 kDa
   

• 

  Other-Anti-Bad (phospho S112) antibody [EPR1891(2)](ab129192)          

     Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D