概述

• 产品名称Anti-Bad抗体[Y208]
     

• 描述

  兔单克隆抗体[Y208] to Bad

• 特异性The antibody does not cross-react with other Bcl-2 members.

• 经测试应用WB,IHC-P,ICC/IF,Flow Cyt,IP

• 种属反应性

  与反应: Mouse, Rat, Dog, Human    
   

• 免疫原

  A synthetic peptide corresponding to residues in the N-term of human Bad.

• 阳性对照    

• Hela cell lysate, human lymphoma.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

   

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Alternative versions available:

  Anti-Bad antibody (Biotin) [Y208] (ab199754)

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

• 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号Y208

• 同种型IgG

• 研究领域

• Cancer

• Cell Death

• Apoptosis

• Metabolism

• Cancer

• Cell Death

• Apoptosis

• Apoptotic Markers

• Bcl 2 family

• Metabolism

• Pathways and Processes

• Metabolism processes

• Apoptosis

• Cancer

• Invasion/microenvironment

• Apoptosis

• Bcl 2 family

• Cell Biology

• Apoptosis

• Intracellular

• Bcl2 Family

Anti-Bad antibody [Y208] 图像

• 

  Western blot - Bad antibody [Y208] (ab32445)          

     Anti-Bad antibody [Y208] (ab32445) at 1/5000 dilution + Hela cell lysate
  
  Predicted band size : 18 kDa
  Observed band size : 23 kDa (why is the actual band size different from the predicted?)
   

• 

  Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208] (ab32445)          

     Immunohistochemical staining of paraffin-embedded human lymphoma using ab32445 at 1/100 dilution.  

• 

  Immunocytochemistry/ Immunofluorescence-Bad antibody [Y208](ab32445)          

     ICC/IF image of ab32445 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32445, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
   

• 

  Flow Cytometry-Bad antibody [Y208](ab32445)          

     Overlay histogram showing MCF-7 cells stained with ab32445 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32445, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in MCF-7 cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.