![Anti-Bad antibody [Y208] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/da45qeqedfg.gif)
![Anti-Bad antibody [Y208] 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/lglbelahqjh.gif)
![Anti-Bad (phospho S112) antibody [EPR1891(2)] 10µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/e4jplit2njy.jpg)
![Anti-Bad (phospho S112) antibody [EPR1891(2)] 40µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/gr5xmevsby3.jpg)
![Anti-Bad (phospho S112) antibody [EPR1891(2)] 100µl](https://yunshiji.oss-cn-shenzhen.aliyuncs.com/202407/25/fvgfihxhicp.jpg)
详细说明
概述
产品名称Anti-Baf180抗体[EPR15860]
描述
兔单克隆抗体[EPR15860] to Baf180
经测试应用IHC-P,WB,ICC/IF
种属反应性
与反应: Mouse, Rat, Human
免疫原
Recombinant fragment within Human Baf180 aa 500-700. The exact sequence is proprietary.
Database link: Q86U86Run BLAST with
Run BLAST with 
阳性对照
WB: Human fetal kidney lysate; HeLa nuclear lysate; IHC-P: Human kidney tissue; ICC/IF: HeLa cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR15860
同种型IgG
研究领域
Epigenetics and Nuclear Signaling
Chromatin Remodeling
Swi / Snf
Epigenetics and Nuclear Signaling
Transcription
Domain Families
HMG Box
Anti-Baf180 antibody [EPR15860] 图像
![Western blot - Anti-Baf180 antibody [EPR15860] (ab196022)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab196022OWB.jpg)
Western blot - Anti-Baf180 antibody [EPR15860] (ab196022)
Predicted band size : 193 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Baf180 knockout HAP1 cell lysate (20 µg)
Lane 3: Human kidney tissue lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab196022 observed at 193 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab196022 was shown to recognize Baf180 when Baf180 knockout samples were used, along with additional cross-reactive bands. Wild-type and Baf180 knockout samples were subjected to SDS-PAGE. ab196022 and ab18058 (loading control to Vinculin) were diluted 1/5000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.![Western blot - Anti-Baf180 antibody [EPR15860] (ab196022)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab196022b-1.jpg)
Western blot - Anti-Baf180 antibody [EPR15860] (ab196022)
Anti-Baf180 antibody [EPR15860] (ab196022) at 1/10000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 193 kDa
Observed band size : 35,193 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
![Western blot - Anti-Baf180 antibody [EPR15860] (ab196022)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab196022b-2.jpg)
Western blot - Anti-Baf180 antibody [EPR15860] (ab196022)
Anti-Baf180 antibody [EPR15860] (ab196022) at 1/10000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) nuclear lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 193 kDa
Observed band size : 193 kDa
Exposure time : 15 seconds5% NFDM/TBST: Blocking and diluting buffer.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [EPR15860] (ab196022)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab196022ihc.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Baf180 antibody [EPR15860] (ab196022)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Baf180 using ab196022 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on Human kidney tissue is observed. Counter stained with Hematoxylin.
Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Baf180 antibody [EPR15860] (ab196022)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab196022ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Baf180 antibody [EPR15860] (ab196022)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling Baf180 with ab196022 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue).
Nuclear staining on HeLa cell line is observed.
-ve control - Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution. The nuclear counter stain is DAPI (blue).
粤公网安备44196802000105号
400-6226-992