详细说明
概述
产品名称Anti-ART3抗体[EPR7220(2)] - C-terminal
描述
兔单克隆抗体[EPR7220(2)] to ART3 - C-terminal
经测试应用WB,IHC-P,IP
种属反应性
与反应: Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human ART3 aa 300 to the C-terminus. The exact sequence is proprietary.
Database link: Q13508阳性对照
Human testis tissue; Human testis lysate
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Protein A purified
克隆单克隆
克隆编号EPR7220(2)
同种型IgG
研究领域
Cell Biology
Other Antibodies
Other Antibodies
Anti-ART3 antibody [EPR7220(2)] - C-terminal 图像
Western blot - Anti-ART3 antibody [EPR7220(2)] (ab192870)
Anti-ART3 antibody [EPR7220(2)] - C-terminal (ab192870) at 1/10000 dilution + Human testis tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 41 kDa (possible mature (processed) protein).Immunoprecipitation - Anti-ART3 antibody [EPR7220(2)] (ab192870)
Immunoprecipitation analysis of Human testis lysate labeling ART3 using ab192870 at 1/20 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody
Note: The band observed at 41 kDa may be due to the removal of a 27 amino acid propeptide (mature form).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ART3 antibody [EPR7220(2)] (ab192870)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ART3 using ab192870 at 1/500 dilution (0.6 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as secondary. Counterstain: Hematoxylin. Negative control also shown.