概述

• 产品名称Anti-APOBEC3B抗体[EPR18138]
     

• 描述

  兔单克隆抗体[EPR18138] to APOBEC3B

• 经测试应用WB

• 种属反应性

  与反应: Human    
   

• 免疫原

  Recombinant fragment within Human APOBEC3B aa 200 to the C-terminus. The exact sequence is proprietary.
  Database link: Q9UH17

       Run BLAST with      Run BLAST with    

• 阳性对照    

• WB:Human APOBEC3B fragment recombinant protein; HepG2, Jurkat and THP-1 whole cell lysates; Human fetal heart lysate; HepaRG-APOBEC3B tetracycline-inducible cell line; HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-); HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line.

• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA

• 
  

• 纯度Protein A purified

• 克隆单克隆

• 克隆编号EPR18138

• 同种型IgG

• 研究领域

• Immunology

• Immune System Diseases

• Antiviral Signaling

• Epigenetics and Nuclear Signaling

• Chromatin Modifying Enzymes

• Deamination

• Epigenetics and Nuclear Signaling

• Chromatin Binding Proteins

• DNA / RNA binding

Anti-APOBEC3B antibody [EPR18138] 图像

• 

  Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)          

     All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution
  
  Lane 1 : Human APOBEC3B fragment recombinant protein
  Lane 2 : Human APOBEC3G fragment recombinant protein
  
  Lysates/proteins at 0.01 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
     

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)          

     Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)          

     Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/2000 dilution + Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)          

     Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + THP-1 (Human monocytic leukemia cell line) whole cell lysate at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
  
  Exposure time : 1 minute    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)          

     Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/1000 dilution + Human fetal heart lysate at 10 µg
  
  Secondary
  Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 46 kDa
  
  
  Exposure time : 3 minutes    

  Blocking/Dilution buffer: 5% NFDM/TBST.

   

• 

  Western blot - Anti-APOBEC3B antibody [EPR18138] (ab184990)          

     All lanes : Anti-APOBEC3B antibody [EPR18138] (ab184990) at 1/10000 dilution
  
  Lane 1 : HepaRG-APOBEC3B tetracycline-inducible cell line (+/-)
  Lane 2 : HCC1143 cell line infected with control (+) and APOBEC3B lentivirus (-)
  Lane 3 : HEK293-APOBEC3B(+GFP) tetracycline-inducible cell line (+/-)
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat Anti-Rabbit IgG (H+L) conjugated to HRP at 1/10000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 35,70 kDa (why is the actual band size different from the predicted?)
     

  Blocking buffer: 5% NFDM/TBST.

  Dilution buffer: 1% NFDM/TBST.

  Exposure time: Panel A: 20 s; Panel B: 1 min.

  Figure provided by Dr. Jenni Nikkila of the Gene Function Lab at The Institute of Cancer Research, London.

  The 35 kDa band represents a known splice variant of APOBEC3B.