小鼠核因子E2相关因子2(Nrf2)ELISA Kit

Mouse Nuclear factor erythroid 2-related factor 2 (Nrf2) ELISA Kit

• 标准曲线:

  These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  ng/ml OD1 OD2 Average Corrected   20 2.648 2.880 2.764 2.627   10 1.966 1.916 1.941 1.804   5 1.357 1.291 1.324 1.187   2.5 0.963 0.945 0.954 0.817   1.25 0.514 0.547 0.531 0.394   0.625 0.358 0.345 0.352 0.215   0.312 0.233 0.239 0.236 0.099   0 0.138 0.135 0.137

   
• 精密度:

  Intra-assay Precision (Precision within an assay): CV%<8%
  Three samples of known concentration were tested twenty times on one plate to assess.
  Inter-assay Precision (Precision between assays): CV%<10%
  Three samples of known concentration were tested in twenty assays to assess.

   
• 线性度:

  To assess the linearity of the assay, samples were spiked with high concentrations of mouse Nrf2 in various matrices diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  		Sample		Serum(n=4)
  1:1		Average %		89
  		Range %		83-94
  1:2		Average %		102
  		Range %		96-107
  1:4		Average %		99
  		Range %		93-104
  1:8		Average %		92
  		Range %		86-99

   
• 回收率:

  The recovery of mouse Nrf2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
  Sample Type		Average % Recovery		Range
  Serum (n=5)		88		82-95
  EDTA plasma (n=4)		95		90-102

   
• 研究领域:
  Cardiovascular