详细说明
概述
产品名称Anti-Apc6抗体[EPR11168]
描述
兔单克隆抗体[EPR11168] to Apc6
经测试应用WB,IHC-P,ICC/IF,IP
种属反应性
与反应: Human
免疫原
Synthetic peptide corresponding to residues in Human Apc6 (UniProt Q13042).
阳性对照
HT-1080, HepG2 and HeLa cell lysates; Human breast carcinoma, gastric carcinoma and kidney tissues; HeLa cells; Immunoprecipitation pellet from HepG2 cell lysate.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR11168
同种型IgG
研究领域
Cell Biology
Proteolysis / Ubiquitin
Proteasome / Ubiquitin
APC/C
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Cell Cycle
Cell Division
Spindle
Anti-Apc6 antibody [EPR11168] 图像
Western blot - Anti-Apc6 antibody [EPR11168] (ab169536)
All lanes : Anti-Apc6 antibody [EPR11168] (ab169536) at 1/1000 dilution
Lane 1 : HT-1080 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size : 71 kDa
Western blot - Anti-Apc6 antibody [EPR11168] (ab169536)
Anti-Apc6 antibody [EPR11168] (ab169536) at 1/1000 dilution + immunoprecipitation pellet from HepG2 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size : 71 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] (ab169536)
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] (ab169536)
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling Apc6 with ab169536 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apc6 antibody [EPR11168] (ab169536)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Apc6 with ab169536 at 1/50 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Apc6 antibody [EPR11168] (ab169536)
Immunofluorescent analysis of HeLa cells labeling Apc6 with ab169536 at 1/100 dilution.