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详细说明
概述
产品名称Anti-ARHGAP25抗体[EPR13233]
描述
兔单克隆抗体[EPR13233] to ARHGAP25
经测试应用WB,IP,ICC/IF
种属反应性
与反应: Human
免疫原
Recombinant fragment within Human ARHGAP25 aa 450-600. The exact sequence is proprietary.
Database link: P42331Run BLAST with
Run BLAST with 
阳性对照
Jurkat, Ramos and Daudi cell lysates; Jurkat cells.
常规说明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA纯度Tissue culture supernatant
克隆单克隆
克隆编号EPR13233
同种型IgG
研究领域
Signal Transduction
Signaling Pathway
G Protein Signaling
Small G Proteins
Regulators
Anti-ARHGAP25 antibody [EPR13233] 图像
![Western blot - Anti-ARHGAP25 [EPR13233] antibody (ab181202)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181202202.JPG)
Western blot - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
All lanes : Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 73 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 [EPR13233] antibody (ab181202)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181202023.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling ARHGAP25 with ab181202 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
![Western blot - Anti-ARHGAP25 [EPR13233] antibody (ab181202)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181202022.JPG)
Western blot - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
Anti-ARHGAP25 antibody [EPR13233] (ab181202) at 1/10000 dilution + Ramos cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 73 kDa
![Immunoprecipitation - Anti-ARHGAP25 [EPR13233] antibody (ab181202)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab181202024.JPG)
Immunoprecipitation - Anti-ARHGAP25 [EPR13233] antibody (ab181202)
Western blot analysis of immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab181202 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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